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Image Search Results
Journal: EMBO Molecular Medicine
Article Title: A Klebsiella pneumoniae antibiotic resistance mechanism that subdues host defences and promotes virulence
doi: 10.15252/emmm.201607336
Figure Lengend Snippet: Immunoblot analysis of IκBα and tubulin levels in lysates of iBMDM cells infected with K. pneumoniae 52145, 52145‐Δ mgrB and 52145‐Δ mgrB Com for the indicated times. Immunoblot analysis of phospho‐ERK (P‐ERK), phospho‐p38 (P‐p38), phospho‐JNK (P‐JNK) and tubulin levels in lysates of iBMDMs cells infected with K. pneumoniae 52145, 52145‐Δ mgrB , 52145‐Δ mgrB Com and for the indicated times. Immunoblot analysis of phospho‐ERK (P‐ERK), phospho‐JNK (P‐JNK) and tubulin levels in lysates of iBMDMs cells infected with K. pneumoniae 52145, 52145‐Δ mgrB , 52145‐Δ pmrC ‐Δ lpxO ‐Δ mgrB ‐Δ pmrF ‐Δ pagP , 52145‐Δ mgrB ‐Δ pmrC , 52145‐Δ mgrB ‐Δ pagP , 52145‐Δ mgrB ‐Δ pmrF and 52145‐Δ mgrB ‐Δ lpxO for 40 min. TNF‐α secretion by iBMDM macrophages stimulated for 6 h with 1 × 10 5 UV‐killed K. pneumoniae 52145, 52145‐Δ mgrB , 52145‐Δ mgrB Com and 52145‐Δ pmrC ‐Δ lpxO ‐Δ mgrB ‐Δ pmrF ‐Δ pagP . *** P < 0.0001; * P = 0.02; versus 52145 determined using two‐way unpaired t ‐test (mean ± SD). Data information: Data are representative of at least three independent experiments. Source data are available online for this figure.
Article Snippet: Immortalised
Techniques: Western Blot, Infection